Answer:
Option C
Explanation:
Resolving limit of microscope is given by
$RL=\frac{\lambda}{2\mu \sin\alpha}$
where, $\mu$ sin $\alpha$ is called numerical aperture [NA]
and $\lambda$ = wavelength of light
$\therefore$ $RL=\frac{\lambda}{2(NA)}$
Hence, if numerical aperture of a microscope is increased , then its limit of resolution is decreased.